Arsenal has been drawn against FC Bayern Munich, while Real Madrid will face defending champions Manchester City in the UEFA Champions League quarter-finals.
In other fixtures, Paris Saint-Germain face Barcelona and Atletico Madrid will take on Borussia Dortmund in the other ties. Pep Guardiola's Manchester City will travel to the Spanish capital for the opening leg on April 6 or 7, with the returning game to be played in Manchester a week later.
Last season, City defeated Madrid in the Champions League semi-finals, winning the competition for the first time after a 1-1 draw at the Santiago Bernabeu and a sensational 4-0 victory at home in the second leg.
The draw produced an intriguing set of fixtures, with Arsenal being rewarded with a match against German champions Bayern after defeating Porto on penalties to advance to the quarter-finals for the first time since 2010.
The Gunners will host the opening leg at Emirates Stadium, where former Tottenham Hotspur star Kane, who has 36 goals in his first season at Bayern, will return to London.
There is the chance of an all-English semi-final, with the winners of the encounter between Arsenal and Bayern playing either City or Real in the last four.
PSG's match against Barcelona will be the latest chapter in their rivalry, with the French club aiming to win the Champions League for the first time in their final season before Kylian Mbappe leaves when his contract expires in June.
Barcelona eliminated PSG in the quarter-finals in 2013 and 2015, before a stunning victory in the last 16 in 2017, when they rebounded from a 4-0 loss away in the first leg with a 6-1 win at the Camp Nou.
The Champions League finals will take place in London on June 1.
Semi-Finals Pairing:
Q4: PSG vs FC Barcelona
Q3: Real Madrid vs Manchester City
Q2: Atletico Madrid vs Borussia Dortmund
Q1: Arsenal vs Bayern Munich
Finals Pairing
Winners of Q2 vs Winners of Q4
Winners of Q1 vs Winners of Q3
